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CD47 Protein, Rat, Recombinant (His)
(Synonyms: CD47 molecule) Copy Product InfoSynonyms: CD47 molecule
Catalog No. TMPY-03093 Copy Product Info
CD47 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is P97829-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $48 | - | In Stock | |
| 10 μg | $75 | - | In Stock | |
| 20 μg | $118 | - | In Stock | |
| 50 μg | $229 | - | In Stock | |
| 100 μg | $386 | - | In Stock | |
| 200 μg | $660 | 7-10 days | 7-10 days | |
| 500 μg | $1,330 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:SDS-PAGE: 98.3%; SEC-HPLC: 94.2%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc , The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml.  |
| Description | CD47 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is P97829-1. |
| Species | Rat |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P97829-1 |
| Construction | A DNA sequence encoding the rat CD47 (P97829-1) (Met1-Lys140) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 19 |
| Protein Purity | > 90 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | CD47 molecule |
| Research Background |
| Molecular Weight | 15.2 kDa (predicted); 27-35 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD47 Protein, Rat, Recombinant (His) chemical structure | CD47 Protein, Rat, Recombinant (His) molecular weight
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