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Anti-Cyclin A1 Antibody (3F206)

😃Good
Catalog No. TMAY-01462
Alias cyclin A1, CT146

Anti-Cyclin A1 Antibody (3F206) is a Mouse antibody targeting Cyclin A1. Anti-Cyclin A1 Antibody (3F206) can be used in ELISA,ICC/IF.

Anti-Cyclin A1 Antibody (3F206)

Anti-Cyclin A1 Antibody (3F206)

😃Good
Catalog No. TMAY-01462Alias cyclin A1, CT146
Anti-Cyclin A1 Antibody (3F206) is a Mouse antibody targeting Cyclin A1. Anti-Cyclin A1 Antibody (3F206) can be used in ELISA,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Mouse Monoclonal

Application: ELISA, ICC/IF

Reactivity: Human
Aliascyclin A1, CT146
Ig Type
Mouse IgG2a
Clone
3F206
Reactivity
Human
Verified Activity
Immunofluorescence staining of Human CCNA1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCNA1 monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.
Application
ELISA,ICC/IF
Recommended Dose
ELISA: 1:5000-1:10000; ICC-IF: 1:100-1:500
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CCNA1 (rh CCNA1; TMPY-01401; NP_003905.1; Met 1-Gln 465). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CCNA1 protein (TMPY-01401)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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