EphB6 Protein, Human, Recombinant (hFc)

Name: EphB6 Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-00789
Rating: 4.5 (1 reviews)

(Synonyms: HEP, EPHB5, EPH receptor B6) Copy Product Info

Synonyms: HEP, EPHB5, EPH receptor B6

Catalog No. TMPY-00789 Copy Product Info

EphB6 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 86.5 kDa and the accession number is O15197.

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	7-10 days	7-10 days
10 µg	$76	7-10 days	7-10 days
20 µg	$118	7-10 days	7-10 days
50 µg	$237	7-10 days	7-10 days
100 µg	$458	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant human EphrinB1 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 0.16-4 μg/ml. 3. Immobilized recombinant human EphrinB2 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 1.28-32 ng/ml.
Description	EphB6 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 86.5 kDa and the accession number is O15197.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	O15197
Construction	The extracellular domain (Met 1-Ser 579) of human EphB6 (NP_004436.1) precursor was fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 17
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	HEP, EPHB5, EPH receptor B6
Research Background	Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. Interestingly, increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration, and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor.

Chemical Properties

Molecular Weight

86.5 kDa (predicted); 100-110 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.