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OX40/TNFRSF4 Protein, Mouse, Recombinant (His & Avi), Biotinylated

(Synonyms: TXGP1L, Txgp1, TNFRSF4, OX40L receptor, OX40, Ly-70, CD134, ACT-135) Copy Product Info

Synonyms: TXGP1L, Txgp1, TNFRSF4, OX40L receptor, OX40, Ly-70, CD134, ACT-135

Catalog No. TMPK-00464 Copy Product Info
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. OX40/TNFRSF4 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 24.2 kDa and the accession number is P47741.

Product Introduction

Bioactivity
Bioactivity
Immobilized Mouse OX40 Ligand, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 4.3μg/ml determined by ELISA.
Description
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. OX40/TNFRSF4 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 24.2 kDa and the accession number is P47741.
Species
Mouse
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP47741
ConstructionVal20-Pro211
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTXGP1L, Txgp1, TNFRSF4, OX40L receptor, OX40, Ly-70, CD134, ACT-135
Research Background
Chemical Properties
Molecular Weight24.2 kDa (predicted). Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: OX40/TNFRSF4 Protein, Mouse, Recombinant (His & Avi), Biotinylated chemical structure | OX40/TNFRSF4 Protein, Mouse, Recombinant (His & Avi), Biotinylated molecular weight