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GDF2 Protein, Human, Recombinant
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Catalog No. TMPK-01697 Copy Product Info
GDF2 Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is Q9UK05.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human GDF-2, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1 ng/ml determined by ELISA (QC Test). Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200). Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4-4 ng/ml (QC Test). |
| Description | GDF2 Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is Q9UK05. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | Q9UK05 |
| Construction | Ser320-Arg429 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 4 mM HCL. Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in 4 mM HCL. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Research Background |
| Molecular Weight | 12.08 kDa (Predicted); 13-15 kDa (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: GDF2 Protein, Human, Recombinant chemical structure | GDF2 Protein, Human, Recombinant molecular weight
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