EphB4 Protein, Human, Recombinant

Catalog No. TMPY-00788 Copy Product Info

EphB4 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.7 kDa and the accession number is P54760.

EphB4 Protein, Human, Recombinant

Catalog No. TMPY-00788

Copy Product Info

EphB4 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.7 kDa and the accession number is P54760.

Pack Size	Price	USA Stock	Global Stock
5 μg	$79	7-10 days	7-10 days
10 μg	$129	7-10 days	7-10 days
20 μg	$208	7-10 days	7-10 days
50 μg	$419	7-10 days	7-10 days
100 μg	$812	7-10 days	7-10 days

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In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Information

Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.
Description	EphB4 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.7 kDa and the accession number is P54760.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P54760
Synonyms	TYRO11,MYK1,HTK,EPH receptor B4
Construction	The mature form of human EphB4 (NP_004435.3) extracellular domain (Met 1-Ala 539) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified. Predicted N terminal: Leu 16
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	57.7 kDa (predicted); 65-70 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.