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Anti-Phospho-NFE2L2 (Ser40) Antibody (6V851)

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Catalog No. TMAH-00935
Alias p-NFE2L2 (Ser40), p-NFE2L2 (S40), Phospho-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40)

Anti-Phospho-NFE2L2 (Ser40) Antibody (6V851) is an antibody targeting Phospho-NFE2L2 (Ser40). Anti-Phospho-NFE2L2 (Ser40) Antibody (6V851) can be used in ELISA, WB, IHC, IF.

Anti-Phospho-NFE2L2 (Ser40) Antibody (6V851)

Anti-Phospho-NFE2L2 (Ser40) Antibody (6V851)

😃Good
Catalog No. TMAH-00935Alias p-NFE2L2 (Ser40), p-NFE2L2 (S40), Phospho-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40)
Anti-Phospho-NFE2L2 (Ser40) Antibody (6V851) is an antibody targeting Phospho-NFE2L2 (Ser40). Anti-Phospho-NFE2L2 (Ser40) Antibody (6V851) can be used in ELISA, WB, IHC, IF.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC, IF

Reactivity: Human
Aliasp-NFE2L2 (Ser40), p-NFE2L2 (S40), Phospho-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40)
Ig Type
Rabbit IgG
Clone
6V851
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in HepG2 whole cell lysate,293 whole cell lysate(treated with Calyculin A or EGF)
-All lanes Phospho-NFE2L2 antibody at 0.8μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 90 KDa
-Observed band size: 90 KDa
2. IHC image of TMAH-00935 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. Immunofluorescence staining of Hela cells with TMAH-00935 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application
ELISA, WB, IHC, IF
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm, cytosol. Nucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundTranscription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes. May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Plays also an important role in the regulation of the innate immune response and antiviral cytosolic DNA sensing. It is a critical regulator of the innate immune response and survival during sepsis by maintaining redox homeostasis and restraint of the dysregulation of proinflammatory signaling pathways like MyD88-dependent and -independent and TNF-alpha signaling. Suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription and the induction of IL6. Binds to the proximity of proinflammatory genes in macrophages and inhibits RNA Pol II recruitment. The inhibition is independent of the NRF2-binding motif and reactive oxygen species level. Represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1 and decreasing responsiveness to STING1 agonists while increasing susceptibility to infection with DNA viruses. Once activated, limits the release of pro-inflammatory cytokines in response to human coronavirus SARS-CoV-2 infection and to virus-derived ligands through a mechanism that involves inhibition of IRF3 dimerization. Also inhibits both SARS-CoV-2 replication, as well as the replication of several other pathogenic viruses including Herpes Simplex Virus-1 and-2, Vaccinia virus, and Zika virus through a type I interferon (IFN)-independent mechanism.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human Phospho-NFE2L2 (S40)
Antigen Species
Human
Gene ID
4780
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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