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Anti-IKBKB Antibody (3T443)
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Catalog No. TMAH-00604
Alias IKBKB
Anti-IKBKB Antibody (3T443) is an antibody targeting IKBKB. Anti-IKBKB Antibody (3T443) can be used in ELISA, WB, IHC, IF.
Anti-IKBKB Antibody (3T443)
Anti-IKBKB Antibody (3T443)
😃Good
Catalog No. TMAH-00604Alias IKBKB
Anti-IKBKB Antibody (3T443) is an antibody targeting IKBKB. Anti-IKBKB Antibody (3T443) can be used in ELISA, WB, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IF Reactivity: Human |
| Alias | IKBKB |
| Ig Type | Rabbit IgG |
| Clone | 3T443 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate -All lanes: IKK beta antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 87 kDa -Observed band size: 87 kDa 2. IHC image of TMAH-00604 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of Hela Cells with TMAH-00604 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IHC, IF |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Membrane raft. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human IKK beta |
| Antigen Species | Human |
| Gene ID | 3551 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cardiovascular, Immunology, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Serine/threonine protein kinase IKBKBNuclear factor NF-kappa-B inhibitor kinase β (NFKBIKB)Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB)Nuclear factor NF-kappa-B inhibitor kinase b (NFKBIKB)NFKBIKBInhibitor of nuclear factor kappa-B kinase subunit βInhibitor of nuclear factor kappa-B kinase subunit betaInhibitor of nuclear factor kappa-B kinase subunit bIKK-βIKKβIKK-betaIKK-BIKKBIKK-2IKK2I-kappa-B-kinase βI-kappa-B-kinase betaI-kappa-B-kinase bI-kappa-B kinase 2 (IKK-2;IKK2)
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