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Anti-TOP2A Antibody (1C511)
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Catalog No. TMAH-01198
Alias TOP2, EC 5.99.1.3, DNA topoisomerase II, DNA topoisomerase 2-alpha, alpha isozyme
Anti-TOP2A Antibody (1C511) is an antibody targeting TOP2A. Anti-TOP2A Antibody (1C511) can be used in ELISA, WB, IHC.
Anti-TOP2A Antibody (1C511)
Anti-TOP2A Antibody (1C511)
😃Good
Catalog No. TMAH-01198Alias TOP2, EC 5.99.1.3, DNA topoisomerase II, DNA topoisomerase 2-alpha, alpha isozyme
Anti-TOP2A Antibody (1C511) is an antibody targeting TOP2A. Anti-TOP2A Antibody (1C511) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human, Mouse |
| Alias | TOP2, EC 5.99.1.3, DNA topoisomerase II, DNA topoisomerase 2-alpha, alpha isozyme |
| Ig Type | Rabbit IgG |
| Clone | 1C511 |
| Reactivity | Human, Mouse |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate -All lanes: TOP2A antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 175, 178, 179, 183 kDa -Observed band size: 175 kDa 2. IHC image of TMAH-01198 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus, nucleoplasm. Nucleus. Nucleus, nucleolus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Topoisomerase II alpha |
| Antigen Species | Human |
| Gene ID | 7153 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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