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Anti-DBF4 Antibody (2Q22)
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Catalog No. TMAH-00332
Alias ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase
Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
Anti-DBF4 Antibody (2Q22)
Anti-DBF4 Antibody (2Q22)
😃Good
Catalog No. TMAH-00332Alias ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase
Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | 198 € | 7-10 days | |
| 100 μL | 330 € | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Alias | ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase |
| Ig Type | Rabbit IgG |
| Clone | 2Q22 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate -All lanes: DBF4 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 77, 27 kDa -Observed band size: 72-95 kDa 2. Immunofluorescence staining of A549 cell with TMAH-00332 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 551-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:2000; IF:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human DBF4 |
| Antigen Species | Human |
| Gene ID | 10926 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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