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Anti-DBF4 Antibody (2Q22) (Synonyms: ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1...)

Catalog No. TMAH-00332 Copy Product Info
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Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA,IF,WB.

Anti-DBF4 Antibody (2Q22)

Copy Product Info
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Catalog No. TMAH-00332
Synonyms ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase

Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA,IF,WB.

Anti-DBF4 Antibody
(2Q22)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2097-10 days7-10 days
100 μL$3487-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA,IF,WB.
SynonymsZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase
Ig Type
Rabbit IgG
Clone
2Q22
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate
-All lanes: DBF4 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 77, 27 kDa
-Observed band size: 72-95 kDa
2. Immunofluorescence staining of A549 cell with TMAH-00332 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 551-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application
Recommended Dose
WB:1:500-1:2000; IF:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus.
Tissue SpecificityHighly expressed in testis and thymus. Expressed also in most cancer cells lines.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundRegulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human DBF4
Antigen Species
Human
Gene Name
DBF4
Gene ID
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling, Cell biology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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