Anti-DBF4 Antibody (2Q22)

Name: Anti-DBF4 Antibody (2Q22)
Brand: TargetMol
SKU: TMAH-00332
Rating: 4.5 (1 reviews)

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Catalog No. TMAH-00332

Alias ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase

Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA,IF,WB.

Anti-DBF4 Antibody (2Q22)

😃Good

Catalog No. TMAH-00332Alias ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase

Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA,IF,WB.

Pack Size	Price	Availability	Quantity
50 μL	$209	7-10 days
100 μL	$348	7-10 days

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Product Introduction

Bioactivity

Description	Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA,IF,WB.
Synonyms	ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase
Ig Type	Rabbit IgG
Clone	2Q22
Reactivity	Human
Verified Activity	1. Western Blot -Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate -All lanes: DBF4 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 77, 27 kDa -Observed band size: 72-95 kDa 2. Immunofluorescence staining of A549 cell with TMAH-00332 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 551-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application	ELISA IF WB
Recommended Dose	WB:1:500-1:2000; IF:1:50-1:200.
Antibody Type	Monoclonal
Subcellular Localization	Nucleus.
Tissue Specificity	Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Construction	Recombinant Antibody
Purification	Affinity-chromatography
Appearance	Liquid
Formulation	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research Background	Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	A synthetic peptide: Human DBF4
Antigen Species	Human
Gene Name	DBF4
Gene ID	10926
Uniprot ID	Q9UBU7
Biology Area	Epigenetics and Nuclear Signaling, Cell biology

Chemical Properties

Stability & Storage

Stability & Storage	Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
Transport	Shipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc