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Synonyms: hypl, angiopoietin-like 3

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $55 | 7-10 days | 7-10 days | |
| 10 µg | $85 | 7-10 days | 7-10 days | |
| 20 µg | $138 | 7-10 days | 7-10 days | |
| 50 µg | $276 | 7-10 days | 7-10 days | |
| 100 µg | $460 | 7-10 days | 7-10 days | |
| 200 µg | $896 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Mouse ANGPTL3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. Loaded Anti-ANGPTL3 Ab., hFc-Avi Tag on ProA-Biosensor can bind Mouse ANGPTL3, His Tag with an affinity constant of 0.84 nM as determined in BLI assay (Gator® Prime). |
| Description | ANGPTL3 Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q9R182. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | Q9R182 |
| Construction | Ser17-Thr455 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | hypl, angiopoietin-like 3 |
| Research Background | ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. |
| Molecular Weight | 51.81 kDa (Predicted); 30-35 kDa, 43-48 kDa and 60-75 kDa (Due to furin cleavage site) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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