Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-TIE2 Antibody (9U826)

😃Good
Catalog No. TMAY-01088
Alias Tie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024

Anti-TIE2 Antibody (9U826) is a Rabbit antibody targeting TIE2. Anti-TIE2 Antibody (9U826) can be used in ELISA(Cap),FCM,ICC/IF.

Anti-TIE2 Antibody (9U826)

Anti-TIE2 Antibody (9U826)

😃Good
Catalog No. TMAY-01088Alias Tie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024
Anti-TIE2 Antibody (9U826) is a Rabbit antibody targeting TIE2. Anti-TIE2 Antibody (9U826) can be used in ELISA(Cap),FCM,ICC/IF.
Pack SizePriceAvailabilityQuantity
20 μL$92 7-10 days
100 μL$268 7-10 days
Bulk & Custom
Add to Cart
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-TIE2 Antibody (9U826) is a Rabbit antibody targeting TIE2. Anti-TIE2 Antibody (9U826) can be used in ELISA(Cap),FCM,ICC/IF.
AliasTie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024
Ig Type
Monoclonal Rabbit IgG
Clone
9U826
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Tie2 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.

2. Flow cytometric analysis of Human TIE2(CD202b) expression on HUVEC cells. Cells were stained with purified anti-Human TIE2(CD202b), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
ELISA(Cap),FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Tie2 / CD202b / TEK (rh Tie2 / CD202b / TEK; TMPY-01064; AAA61139.1; Met1-Lys745).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundTEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/FITC/PE
Antibody Types Available
4
Antigen Details
Immunogen
Recombinant Human Tie2 / CD202b / TEK protein (TMPY-01064)
Antigen Species
Human
Biology Area
Receptor Tyrosine Kinases (RTKs), Cancer Drug Targets, Hemangioblast Markers
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-TIE2 Antibody (9U826) | purchase Anti-TIE2 Antibody (9U826) | Anti-TIE2 Antibody (9U826) cost | order Anti-TIE2 Antibody (9U826)