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Anti-TIE2 Antibody (9U826)
😃Good
Catalog No. TMAY-01088
Alias Tie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024
Anti-TIE2 Antibody (9U826) is a Rabbit antibody targeting TIE2. Anti-TIE2 Antibody (9U826) can be used in ELISA(Cap),FCM,ICC/IF.
Anti-TIE2 Antibody (9U826)
Anti-TIE2 Antibody (9U826)
😃Good
Catalog No. TMAY-01088Alias Tie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024
Anti-TIE2 Antibody (9U826) is a Rabbit antibody targeting TIE2. Anti-TIE2 Antibody (9U826) can be used in ELISA(Cap),FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA(Cap), FCM, ICC/IF Reactivity: Human |
| Alias | Tie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024 |
| Ig Type | Rabbit IgG |
| Clone | 9U826 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Tie2 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm. 2. Flow cytometric analysis of Human TIE2(CD202b) expression on HUVEC cells. Cells were stained with purified anti-Human TIE2(CD202b), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA(Cap),FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Tie2 / CD202b / TEK (rh Tie2 / CD202b / TEK; TMPY-01064; AAA61139.1; Met1-Lys745). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature. |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Protein: Human Tie2 / CD202b / TEK protein (TMPY-01064) |
| Antigen Species | Human |
| Biology Area | Receptor Tyrosine Kinases (RTKs), Cancer Drug Targets, Hemangioblast Markers |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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