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Notch 1 Protein, Mouse, Recombinant (His)

(Synonyms: Tan1, Notch1, notch 1, N1, Mis6, lin-12, 9930111A19Rik) Copy Product Info

Synonyms: Tan1, Notch1, notch 1, N1, Mis6, lin-12, 9930111A19Rik

Catalog No. TMPY-02663 Copy Product Info
Notch 1 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 55 kDa and the accession number is Q01705-1.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$687-10 days7-10 days
10 μg$1087-10 days7-10 days
20 μg$1787-10 days7-10 days
50 μg$3577-10 days7-10 days
100 μg$6297-10 days7-10 days
200 μg$1,0907-10 days7-10 days
500 μg$2,7007-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.
Description
Notch 1 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 55 kDa and the accession number is Q01705-1.
Species
Mouse
Expression System
Baculovirus Insect Cells
TagC-His
Accession NumberQ01705-1
ConstructionA DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed,with a C-terminal polyhistidine tag. Predicted N terminal: Ala 19
Protein Purity
> 82 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTan1, Notch1, notch 1, N1, Mis6, lin-12, 9930111A19Rik
Research Background
Chemical Properties
Molecular Weight55 kDa (predicted); 80 kDa (reducing conditions)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Notch 1 Protein, Mouse, Recombinant (His) chemical structure | Notch 1 Protein, Mouse, Recombinant (His) molecular weight