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Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) is an antibody targeting Acetyl-Histone H2A type 1-B/E (Lys9). Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) can be used in ELISA, ICC, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, ICC, IF Reactivity: Human |
Alias | Histone H2AK9-acetylated, Histone H2A/m, Histone H2A/a, Histone H2A.2, Histone H2A type 1-B/E, HIST1H2AE, HIST1H2AB, H2AK9ac, H2AFM, H2AFA, AND, Ac-Histone H2A type 1-B/E (Lys9), Ac-Histone H2A type 1-B/E (K9), Ac-H2AK9, Acetyl-Histone H2A type 1-B/E (K9) |
Ig Type | Rabbit IgG |
Clone | 9N784 |
Reactivity | Human |
Verified Activity | 1. Immunocytochemistry analysis of TMAH-00007 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 2. Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with TMAH-00007 at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, ICC, IF |
Recommended Dose | ICC:1:50-1:500; IF:1:30-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. Chromosome. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide |
Antigen Species | Human |
Gene ID | 3012 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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