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Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784)

😃Good
Catalog No. TMAH-00007
Alias Histone H2AK9-acetylated, Histone H2A/m, Histone H2A/a, Histone H2A.2, Histone H2A type 1-B/E, HIST1H2AE, HIST1H2AB, H2AK9ac, H2AFM, H2AFA, AND, Ac-Histone H2A type 1-B/E (Lys9), Ac-Histone H2A type 1-B/E (K9), Ac-H2AK9, Acetyl-Histone H2A type 1-B/E (K9)

Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) is an antibody targeting Acetyl-Histone H2A type 1-B/E (Lys9). Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) can be used in ELISA, ICC, IF.

Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784)

Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784)

😃Good
Catalog No. TMAH-00007Alias Histone H2AK9-acetylated, Histone H2A/m, Histone H2A/a, Histone H2A.2, Histone H2A type 1-B/E, HIST1H2AE, HIST1H2AB, H2AK9ac, H2AFM, H2AFA, AND, Ac-Histone H2A type 1-B/E (Lys9), Ac-Histone H2A type 1-B/E (K9), Ac-H2AK9, Acetyl-Histone H2A type 1-B/E (K9)
Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) is an antibody targeting Acetyl-Histone H2A type 1-B/E (Lys9). Anti-Acetyl-Histone H2A type 1-B/E (Lys9) Antibody (9N784) can be used in ELISA, ICC, IF.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Monoclonal

Application: ELISA, ICC, IF

Reactivity: Human
AliasHistone H2AK9-acetylated, Histone H2A/m, Histone H2A/a, Histone H2A.2, Histone H2A type 1-B/E, HIST1H2AE, HIST1H2AB, H2AK9ac, H2AFM, H2AFA, AND, Ac-Histone H2A type 1-B/E (Lys9), Ac-Histone H2A type 1-B/E (K9), Ac-H2AK9, Acetyl-Histone H2A type 1-B/E (K9)
Ig Type
Rabbit IgG
Clone
9N784
Reactivity
Human
Verified Activity
1. Immunocytochemistry analysis of TMAH-00007 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
2. Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with TMAH-00007 at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application
ELISA, ICC, IF
Recommended Dose
ICC:1:50-1:500; IF:1:30-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus. Chromosome.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide
Antigen Species
Human
Gene ID
3012
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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