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Anti-CD80 Polyclonal Antibody
Catalog No. TMAB-04075 Copy Product Info
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Anti-CD80 Polyclonal Antibody is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody can be used in WB,ELISA.
Anti-CD80 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-04075
Anti-CD80 Polyclonal Antibody is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody can be used in WB,ELISA.
Anti-CD80 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-CD80 Polyclonal Antibody is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody can be used in WB,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Human HL-60 cell lysates Primary: Anti-CD80 (TMAB-04075) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 68 kDa 2. Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse CD80 Antibody, the EC50 is 31.10 ng/mL. 3. Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse CD80 Antibody, the EC50 is 36.63 ng/mL. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Expressed on activated B-cells, macrophages and dendritic cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. |
| Immunogen | Recombinant Protein: mouse CD80 protein |
| Antigen Species | Mouse |
| Gene Name | CD80 |
| Gene ID | |
| Protein Name | T-lymphocyte activation antigen CD80 |
| Uniprot ID | |
| Function | Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. |
| Molecular Weight | Theoretical: 32 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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