Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, IF

Reactivity: Human

Rabbit IgG

2Y782

Human

1. IHC image of TMAH-00849 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
2. Immunofluorescence staining of MCF-7 cell with TMAH-00849 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ELISA, IHC, IF

IHC:1:50-1:200; IF:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human PAH

Human

5053

Metabolism, Signal transduction