IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | In stock | $ 245.00 | |
100 μg | 5 days | $ 435.00 | |
200 μg | 5 days | $ 770.00 | |
500 μg | 5 days | $ 2,530.00 |
Biological Information |
1. Immobilized Human IL23 Receptor hFc at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His, the EC50 of Mouse IL-23 His is 50-250 ng/mL.
2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4 ng/mL.
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Description | IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | Q9EQ14&P43432 |
Synonyms | IL-23, p19 |
Construction | The Mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Mouse IL23 heterodimer was purified. |
Protein Purity |
> 95 % as determined by SDS-PAGE
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Molecular Weight | 58.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-23 Protein, Mouse, Recombinant (His) IL-23 p19 IL 23 IL23 recombinant recombinant-proteins proteins protein