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IL-23 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-03526
Synonyms: IL-23, p19

IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432.

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IL-23 Protein, Mouse, Recombinant (His)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 245.00
100 μg 5 days $ 435.00
200 μg 5 days $ 770.00
500 μg 5 days $ 2,530.00
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Biological Description
Technical Params
Product Properties
Biological Information 1. Immobilized Human IL23 Receptor hFc at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His, the EC50 of Mouse IL-23 His is 50-250 ng/mL. 2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4 ng/mL.

Description IL-23 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.3 kDa and the accession number is Q9EQ14&P43432.
Species Mouse
Expression System HEK293 Cells
Tag C-His
Accession Number Q9EQ14&P43432
Synonyms IL-23, p19
Construction The Mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Mouse IL23 heterodimer was purified.
Protein Purity > 95 % as determined by SDS-PAGE

Molecular Weight 58.3 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

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Keywords

IL-23 Protein, Mouse, Recombinant (His) IL-23 p19 IL 23 IL23 recombinant recombinant-proteins proteins protein

 

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