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IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi)
(Synonyms: Interleukin-1 β, Interleukin-1 beta, IL-1β, IL1F2, IL-1BETA, IL-1B, IL1B, IL-1 β/IL-1F2, IL-1 β, IL-1 beta) Copy Product InfoSynonyms: Interleukin-1 β, Interleukin-1 beta, IL-1β, IL1F2, IL-1BETA, IL-1B, IL1B, IL-1 β/IL-1F2, IL-1 β, IL-1 beta
Catalog No. TMPK-00161 Copy Product Info
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) is expressed in E. coli expression system with C-His-Avi tag. The predicted molecular weight is 20.1 kDa and the accession number is P01584.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $51 | - | In Stock | |
| 10 µg | $80 | - | In Stock | |
| 20 µg | $129 | - | In Stock | |
| 50 µg | $248 | 7-10 days | 7-10 days | |
| 100 µg | $418 | - | In Stock | |
| 200 µg | $757 | 7-10 days | 7-10 days | |
| 500 µg | $1,670 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA (QC Test).
2. Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay. |
| Description | Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) is expressed in E. coli expression system with C-His-Avi tag. The predicted molecular weight is 20.1 kDa and the accession number is P01584. |
| Species | Human |
| Expression System | E. coli |
| Tag | C-His-Avi |
| Accession Number | P01584 |
| Construction | Ala117-Ser269 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC  , |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | Interleukin-1 β, Interleukin-1 beta, IL-1β, IL1F2, IL-1BETA, IL-1B, IL1B, IL-1 β/IL-1F2, IL-1 β, IL-1 beta |
| Research Background | Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
| Molecular Weight | 20.1 kDa (predicted) same as Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) chemical structure | IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) molecular weight
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