Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 418.00 | |
500 μg | 5 days | $ 1,670.00 | |
1 mg | 5 days | $ 2,800.00 |
Biological Information | 1. Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA (QC Test). 2. Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay. |
Description | Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
Species | Human |
Expression System | E. coli |
Tag | C-His-Avi |
Accession Number | P01584 |
Synonyms | Interleukin-1 beta, IL-1 β, IL-1β, IL1F2, IL-1BETA, Interleukin-1 β, IL-1B, IL1B, IL-1 β/IL-1F2, IL-1 beta |
Construction | Recombinant Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus. It contains Ala117-Ser269.[Accession |P01584] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
Molecular Weight | The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi) IL-1 b Interleukin-1 beta IL-1 β IL-1β IL1F2 IL-1BETA Interleukin-1 β IL-1B IL1B IL-1 β/IL-1F2 IL-1 beta Interleukin-1 b recombinant recombinant-proteins proteins protein