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Anti-Alpha-fetoprotein Antibody (5G216)
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Catalog No. TMAB-00104
Anti-Alpha-fetoprotein Antibody (5G216) is a Mouse antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (5G216) can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-Alpha-fetoprotein Antibody (5G216)
Anti-Alpha-fetoprotein Antibody (5G216)
😃Good
Catalog No. TMAB-00104
Anti-Alpha-fetoprotein Antibody (5G216) is a Mouse antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (5G216) can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Alpha-fetoprotein Antibody (5G216) is a Mouse antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (5G216) can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| Clone | 5G216 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/mL. 2. 25 μg total protein per Lane of various lysates probed with AFp (A2) monoclonal antibody, unconjugated (TMAB-00104) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 3. (Negative control) paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFp (A2) Monoclonal Antibody, Unconjugated (TMAB-00104) at 1:500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. (Negative control) paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFp (A2) Monoclonal Antibody, Unconjugated (TMAB-00104) at 1:500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFp (A2) Monoclonal Antibody, Unconjugated (TMAB-00104) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| Research Background | Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. |
| Immunogen | AFP protein |
| Biology Area | Tumor antigens,Serum Proteins,Liver development,Cardiovascular ELISA kits |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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