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Anti-NUDT5 Antibody (4D504)
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Catalog No. TMAH-00836
Alias YSAH1, YSA1H, YSA1, nudix (nucleoside diphosphate linked moiety X)-type motif 5, hYSAH1
Anti-NUDT5 Antibody (4D504) is an antibody targeting NUDT5. Anti-NUDT5 Antibody (4D504) can be used in ELISA, WB, FCM.
Anti-NUDT5 Antibody (4D504)
Anti-NUDT5 Antibody (4D504)
😃Good
Catalog No. TMAH-00836Alias YSAH1, YSA1H, YSA1, nudix (nucleoside diphosphate linked moiety X)-type motif 5, hYSAH1
Anti-NUDT5 Antibody (4D504) is an antibody targeting NUDT5. Anti-NUDT5 Antibody (4D504) can be used in ELISA, WB, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, FCM Reactivity: Human |
| Alias | YSAH1, YSA1H, YSA1, nudix (nucleoside diphosphate linked moiety X)-type motif 5, hYSAH1 |
| Ig Type | Rabbit IgG |
| Clone | 4D504 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate -All lanes: NUDT5 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 25 kDa -Observed band size: 36 kDa 2. Overlay Peak curve showing HepG2 cells stained with TMAH-00836 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, FCM |
| Recommended Dose | WB:1:500-1:2000; FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate. In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human NUDT5 |
| Antigen Species | Human |
| Gene ID | 11164 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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