Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC, IF, FCM

Reactivity: Human

IgG2b

2L342

Human

1. Western Blot
-Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate
-All lanes: PD-L1 antibody at 1:2500
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 34, 21 kDa
-Observed band size: 55 kDa
2. Western Blot
-Positive WB detected in: Hela whole cell lysate
-All lanes: PD-L1 antibody at 1:1000, 1:2000, 1:4000
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 34, 21 kDa
-Observed band size: 55 kDa
3. IHC image of TMAH-00870 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4. IHC image of TMAH-00870 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
5. Immunofluorescence staining of 293 cells with TMAH-00870 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
6. Immunofluorescence staining of A549 cells with TMAH-00870 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
7. Immunofluorescence staining of Hela cells with TMAH-00870 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
8. Overlay histogram showing 293 cells stained with TMAH-00870 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
9. Overlay histogram showing A549 cells stained with TMAH-00870 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
10. Overlay histogram showing Hela cells stained with TMAH-00870 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, IF, FCM

Monoclonal

Unconjugated

Recombinant Protein: Human Programmed cell death 1 ligand 1 Protein (19-238AA)

Human

29126

Immunology