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Anti-Carboxypeptidase M Antibody (6K425) (Synonyms: E030045M14Rik, carboxypeptidase M, AA589379, 5730456K23Ri...)

Catalog No. TMAY-01778 Copy Product Info
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Anti-Carboxypeptidase M Antibody (6K425) is a Rabbit antibody targeting Carboxypeptidase M. Anti-Carboxypeptidase M Antibody (6K425) can be used in ELISA,IP,WB.

Anti-Carboxypeptidase M Antibody (6K425)

Copy Product Info
🥰Excellent
Catalog No. TMAY-01778
Synonyms E030045M14Rik, carboxypeptidase M, AA589379, 5730456K23Rik, 1110060I01Rik

Anti-Carboxypeptidase M Antibody (6K425) is a Rabbit antibody targeting Carboxypeptidase M. Anti-Carboxypeptidase M Antibody (6K425) can be used in ELISA,IP,WB.

Anti-Carboxypeptidase M Antibody
(6K425)
Pack SizePriceUSA StockGlobal StockQuantity
100 μL$2697-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Carboxypeptidase M Antibody (6K425) is a Rabbit antibody targeting Carboxypeptidase M. Anti-Carboxypeptidase M Antibody (6K425) can be used in ELISA,IP,WB.
SynonymsE030045M14Rik, carboxypeptidase M, AA589379, 5730456K23Rik, 1110060I01Rik
Ig Type
Rabbit IgG
Clone
6K425
Reactivity
Human
Specificity
Human Carboxypeptidase M
Verified Activity
1. Anti-CPM rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:51 kDa.
-Observed band size:55 kDa(We are unsure as to the identity of these extra bands.)
2. CPM was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-CPM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-CPM rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution.
-Developed using the DAB staining technique.
-Performed under reducing conditions.
-Predicted band size: 55 kDa.
-Observed band size: 55 kDa
Application
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Carboxypeptidase M / CPM (rh Carboxypeptidase M / CPM; TMPY-01313; NP_938079.1; Met 1-His 422).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCarboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human Carboxypeptidase M / CPM Protein (TMPY-01313)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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