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Anti-Carboxypeptidase M Antibody (6K425)
😃Good
Catalog No. TMAY-01778
Alias E030045M14Rik, carboxypeptidase M, AA589379, 5730456K23Rik, 1110060I01Rik
Anti-Carboxypeptidase M Antibody (6K425) is a Rabbit antibody targeting Carboxypeptidase M. Anti-Carboxypeptidase M Antibody (6K425) can be used in WB,ELISA,IP.
Anti-Carboxypeptidase M Antibody (6K425)
Anti-Carboxypeptidase M Antibody (6K425)
😃Good
Catalog No. TMAY-01778Alias E030045M14Rik, carboxypeptidase M, AA589379, 5730456K23Rik, 1110060I01Rik
Anti-Carboxypeptidase M Antibody (6K425) is a Rabbit antibody targeting Carboxypeptidase M. Anti-Carboxypeptidase M Antibody (6K425) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Carboxypeptidase M Antibody (6K425) is a Rabbit antibody targeting Carboxypeptidase M. Anti-Carboxypeptidase M Antibody (6K425) can be used in WB,ELISA,IP. |
| Alias | E030045M14Rik, carboxypeptidase M, AA589379, 5730456K23Rik, 1110060I01Rik |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6K425 |
| Reactivity | Human |
| Verified Activity | 1. Anti-CPM rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:51 kDa. -Observed band size:55 kDa(We are unsure as to the identity of these extra bands.) 2. CPM was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-CPM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CPM rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 55 kDa. -Observed band size: 55 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carboxypeptidase M / CPM (rh Carboxypeptidase M / CPM; TMPY-01313; NP_938079.1; Met 1-His 422). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Carboxypeptidase M / CPM Protein (TMPY-01313) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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