PD-L1 Protein, Human, Recombinant (Flag)

Catalog No. TMPJ-00183 Copy Product Info

PD-L1 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 35-40 KDa and the accession number is Q9NZQ7.

PD-L1 Protein, Human, Recombinant (Flag)

Catalog No. TMPJ-00183

Copy Product Info

PD-L1 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 35-40 KDa and the accession number is Q9NZQ7.

Pack Size	Price	USA Stock	Global Stock
5 μg	$51	-	In Stock
10 μg	$79	-	In Stock
20 μg	$113	2-4 weeks	2-4 weeks
50 μg	$183	2-4 weeks	2-4 weeks
100 μg	$335	2-4 weeks	2-4 weeks
200 μg	$615	2-4 weeks	2-4 weeks
500 μg	$1,390	2-4 weeks	2-4 weeks
1 mg	$1,980	2-4 weeks	2-4 weeks

Add to Cart

Add to Quotation

For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Biological Activity	1. Loaded Human PD-1-Fc on Protein A Biosensor, can bind Human PD-L1-Flag with an affinity constant of 0.19 uM as determined in BLI assay. (Regularly tested) 2. Immobilized Human PD-L1-Flag at 2μg/ml (100 μl/well)can bind Anti-Human PDL1 mAb-Fc. The ED50 of Anti-Human PDL1 mAb-Fc is 14.68 ng/ml. (Regularly tested)
Description	PD-L1 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 35-40 KDa and the accession number is Q9NZQ7.
Species	Human
Expression System	HEK293 Cells
Tag	C-Flag
Accession Number	Q9NZQ7
Synonyms	Programmed Death Ligand 1,Programmed Cell Death 1 Ligand 1,PD-L1,PDL1,PDCD1LG1,PDCD1L1,PDCD1 Ligand 1,CD274,B7-H1,B7H1,B7 Homolog 1
Amino Acid	Phe19-Thr239
Construction	Phe19-Thr239
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	35-40 KDa (reducing condition)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.