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Anti-N Antibody (5A367) is an antibody targeting N. Anti-N Antibody (5A367) can be used in ELISA, WB, GICA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, GICA Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Alias | Nucleocapsid, NP, NC |
Ig Type | Mouse scFv fusion with human IgG1 Fc |
Clone | 5A367 |
Reactivity | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Verified Activity | 1. Western Blot -Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli SARS-CoV-2 nucleocapsid antibody at 1:1000 (TMAH-00800) -Secondary: Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution -Predicted band size: 48 kDa -Observed band size: 55 kDa 2. The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml. 3. In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (TMAH-00800) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good. 4. ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (TMAH-00800) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1. 5. The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody. 6. The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody. 7. The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody. |
Application | ELISA, WB, GICA |
Recommended Dose | ELISA:1:10000-1:50000; WB:1:500-1:5000; GICA:1:1000-1:10000. |
Antibody Type | Monoclonal |
Subcellular Localization | Virion. Host cytoplasm. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3. |
Conjucates | Unconjugated |
Others Formats | Biotin/FITC/HRP |
Antibody Types Available | 4 |
Immunogen | Recombinant Protein: Human Novel Coronavirus NucleoProtein (N) (1-419aa) |
Antigen Species | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Uniprot ID | |
Biology Area | Microbiology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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