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Anti-NeuroD1 Antibody (6V209)
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Catalog No. TMAH-00812
Alias Neurogenic differentiation factor 1, NeuroD 1, NeuroD, NEUROD, Class A basic helix-loop-helix protein 3, BHLHA3
Anti-NeuroD1 Antibody (6V209) is an antibody targeting NeuroD1. Anti-NeuroD1 Antibody (6V209) can be used in ELISA, IF, FC.
Anti-NeuroD1 Antibody (6V209)
Anti-NeuroD1 Antibody (6V209)
😃Good
Catalog No. TMAH-00812Alias Neurogenic differentiation factor 1, NeuroD 1, NeuroD, NEUROD, Class A basic helix-loop-helix protein 3, BHLHA3
Anti-NeuroD1 Antibody (6V209) is an antibody targeting NeuroD1. Anti-NeuroD1 Antibody (6V209) can be used in ELISA, IF, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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| Description | Anti-NeuroD1 Antibody (6V209) is an antibody targeting NeuroD1. Anti-NeuroD1 Antibody (6V209) can be used in ELISA, IF, FC. |
| Alias | Neurogenic differentiation factor 1, NeuroD 1, NeuroD, NEUROD, Class A basic helix-loop-helix protein 3, BHLHA3 |
| Ig Type | Rabbit IgG |
| Clone | 6V209 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of A549 with TMAH-00812 at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 522-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing SH-SY5Y cells stained with TMAH-00812 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, IF, FC |
| Recommended Dose | IF:1:50-1:200; FC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human NEUROD1 |
| Antigen Species | Human |
| Gene ID | 4760 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Cardiovascular, Metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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