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Anti-Tissue Factor Antibody (2C735)

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Catalog No. TMAY-02497
Alias TFA, TF, coagulation factor III (thromboplastin, tissue factor), coagulation factor III, coagulation factor 3, CD142

Anti-Tissue Factor Antibody (2C735) is a Mouse antibody targeting Tissue Factor. Anti-Tissue Factor Antibody (2C735) can be used in ELISA,FCM,ICC/IF.

Anti-Tissue Factor Antibody (2C735)

Anti-Tissue Factor Antibody (2C735)

😃Good
Catalog No. TMAY-02497Alias TFA, TF, coagulation factor III (thromboplastin, tissue factor), coagulation factor III, coagulation factor 3, CD142
Anti-Tissue Factor Antibody (2C735) is a Mouse antibody targeting Tissue Factor. Anti-Tissue Factor Antibody (2C735) can be used in ELISA,FCM,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Tissue Factor Antibody (2C735) is a Mouse antibody targeting Tissue Factor. Anti-Tissue Factor Antibody (2C735) can be used in ELISA,FCM,ICC/IF.
AliasTFA, TF, coagulation factor III (thromboplastin, tissue factor), coagulation factor III, coagulation factor 3, CD142
Ig Type
Monoclonal Mouse IgG2a
Clone
2C735
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human F3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human F3 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cell membrane and cytoplasm.

2. Flow cytometric analysis of Human F3(CD142) expression on A431 cells. Cells were stained with purified anti-Human F3(CD142), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
ELISA,FCM,ICC/IF
Recommended Dose
ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human F3 / Tissue factor / CD142 (rh F3 / Tissue factor / CD142; TMPY-02530; P13726; Met 1-Glu 251). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundTissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/FITC/PE
Antibody Types Available
4
Antigen Details
Immunogen
Recombinant Human F3 / Tissue factor / CD142 protein (TMPY-02530)
Antigen Species
Human
Biology Area
Serine Proteases and Regulators
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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