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Anti-HBsAg Polyclonal Antibody

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Catalog No. TMAB-00835
Alias S, Major surface antigen, L-HBsAg, LHB, Large surface protein, Large S protein, L glycoprotein, HBSAG_HBVC5

Anti-HBsAg Polyclonal Antibody is a Goat antibody targeting HBsAg. Anti-HBsAg Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-HBsAg Polyclonal Antibody

Anti-HBsAg Polyclonal Antibody

😃Good
Catalog No. TMAB-00835Alias S, Major surface antigen, L-HBsAg, LHB, Large surface protein, Large S protein, L glycoprotein, HBSAG_HBVC5
Anti-HBsAg Polyclonal Antibody is a Goat antibody targeting HBsAg. Anti-HBsAg Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-HBsAg Polyclonal Antibody is a Goat antibody targeting HBsAg. Anti-HBsAg Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
AliasS, Major surface antigen, L-HBsAg, LHB, Large surface protein, Large S protein, L glycoprotein, HBSAG_HBVC5
Ig Type
IgG
Reactivity
Human,HBV (predicted:Bee,Hepatitis B virus)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Hepatitis B virus infected human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HBSAg) Polyclonal Antibody, Unconjugated (TMAB-00835) at 1:200 overnight at 4°C, followed by A594 conjugated Rabbit Anti-Goat IgG H&L secondary antibody incubation and staining.
2. Measured by its binding ability in a indirect ELISA. Immobilized recombinant HBSAg protein at 2 μg/mL (100 μL/well) can bind  Goat Anti-Human HBSAg Antibody, the EC50 is 29.14 ng/mL.
3. Sample:
Lane 1: Recombinant HBSAg protein
Primary: Anti-HBSAg (TMAB-00835) at 1/1000 dilution
Secondary: IRDye800CW Donkey anti-Goat IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 20 kDa
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesGoat
Subcellular LocalizationVirion membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M PBS (pH7.4) with 0.02% Proclin300.
Antigen Details
Immunogen
Recombinant Protein: HBsAg protein
Biology Area
Hepatitis B
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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