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Anti-HBsAg Polyclonal Antibody
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Catalog No. TMAB-00835
Alias S, Major surface antigen, L-HBsAg, LHB, Large surface protein, Large S protein, L glycoprotein, HBSAG_HBVC5
Anti-HBsAg Polyclonal Antibody is a Goat antibody targeting HBsAg. Anti-HBsAg Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-HBsAg Polyclonal Antibody
Anti-HBsAg Polyclonal Antibody
😃Good
Catalog No. TMAB-00835Alias S, Major surface antigen, L-HBsAg, LHB, Large surface protein, Large S protein, L glycoprotein, HBSAG_HBVC5
Anti-HBsAg Polyclonal Antibody is a Goat antibody targeting HBsAg. Anti-HBsAg Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HBsAg Polyclonal Antibody is a Goat antibody targeting HBsAg. Anti-HBsAg Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Alias | S, Major surface antigen, L-HBsAg, LHB, Large surface protein, Large S protein, L glycoprotein, HBSAG_HBVC5 |
| Ig Type | IgG |
| Reactivity | Human,HBV (predicted:Bee,Hepatitis B virus) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Hepatitis B virus infected human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HBSAg) Polyclonal Antibody, Unconjugated (TMAB-00835) at 1:200 overnight at 4°C, followed by A594 conjugated Rabbit Anti-Goat IgG H&L secondary antibody incubation and staining. 2. Measured by its binding ability in a indirect ELISA. Immobilized recombinant HBSAg protein at 2 μg/mL (100 μL/well) can bind Goat Anti-Human HBSAg Antibody, the EC50 is 29.14 ng/mL. 3. Sample: Lane 1: Recombinant HBSAg protein Primary: Anti-HBSAg (TMAB-00835) at 1/1000 dilution Secondary: IRDye800CW Donkey anti-Goat IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 20 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Goat |
| Subcellular Localization | Virion membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| Immunogen | Recombinant Protein: HBsAg protein |
| Biology Area | Hepatitis B |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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