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Anti-COMT Antibody (6P98) is an antibody targeting COMT. Anti-COMT Antibody (6P98) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
Alias | HEL-S-98n, catechol-O-methyltransferase |
Ig Type | Rabbit IgG |
Clone | 6P98 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate -All lanes: COMT Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 31, 25 kDa -Observed band size: 31 kDa 2. IHC image of TMAH-00292 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | [Isoform Soluble]: Cytoplasm.; [Isoform Membrane-bound]: Cell membrane; Single-pass type II membrane protein; Extracellular side. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human COMT |
Antigen Species | Human |
Gene ID | 1312 |
Uniprot ID | |
Biology Area | Neuroscience, Cancer, Metabolism, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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