CD20 Protein, Human, Recombinant (Flag)

Name: CD20 Protein, Human, Recombinant (Flag)
Brand: TargetMol
SKU: TMPJ-00184
Rating: 4.5 (1 reviews)

(Synonyms: MS4A1, Membrane-spanning 4-domains subfamily A member 1, Leukocyte surface antigen Leu-16, CD20, Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20) Copy Product Info

Synonyms: MS4A1, Membrane-spanning 4-domains subfamily A member 1, Leukocyte surface antigen Leu-16, CD20, Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20

Catalog No. TMPJ-00184 Copy Product Info

CD20 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 33-37 kDa and the accession number is P11836.

Pack Size	Price	USA Stock	Global Stock
10 µg	$465	7-10 days	7-10 days
20 µg	$746	7-10 days	7-10 days
50 µg	$1,400	7-10 days	7-10 days
100 µg	$2,280	7-10 days	7-10 days
200 µg	$3,790	7-10 days	7-10 days
500 µg	$7,400	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Loaded Anti-Human CD20 mAb-Fc on AHC Biosensor, can bind Human CD20-Flag with an affinity constant of 3.47 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Human CD20-Flag at 2μg/ml (100 μl/well) can bind Anti-Human CD20 mAb . The ED50 of Anti-Human CD20 mAb is 11.09 ng/ml. (Regularly tested)
Description	CD20 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 33-37 kDa and the accession number is P11836.
Species	Human
Expression System	HEK293 Cells
Tag	C-Flag
Accession Number	P11836
Amino Acid	Met1-Pro297
Construction	Met1-Pro297
Protein Purity	Greater than 70% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 0.06% DDM, 0.012% CHS, 10% Glycerol, pH 7.4.
Synonyms	MS4A1, Membrane-spanning 4-domains subfamily A member 1, Leukocyte surface antigen Leu-16, CD20, Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20
Research Background	CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG.

Chemical Properties

Molecular Weight

33-37 kDa (reducing condition)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.