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F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-HRP is a HRP-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-HRP can be used in ELISA,IHC-F,IHC-P,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $415 | 7-10 days | |
1 mL | $3,785 | 7-10 days |
Description | F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-HRP is a HRP-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-Mouse IgG H&L Secondary Antibody-HRP can be used in ELISA,IHC-F,IHC-P,WB. |
Synonyms | F(ab)2 Fragment Goat Anti-Mouse IgG(H+L), F(ab)2 Fragment Goat Anti-Mouse IgG H&L/HRP, F(ab)2 Fragment Goat Anti-Mouse IgG H&L (HRP) |
Ig Type | IgG |
Reactivity | Mouse |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, An HRP-conjugated secondary (TMAB-02063H, 1:300 dilution) was used for 20min at 37℃,DAB was used as the chromogen.
2. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLP-1) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, An HRP-conjugated secondary (TMAB-02063H, 1:300 dilution) was used for 20min at 37℃,DAB was used as the chromogen. ![]() ![]() |
Application | |
Recommended Dose | IHC-P: 1:1000; WB: 1:1000-20000; IHC-F: 1:100-1000; ELISA: 1:1000-20000 |
Antibody Type | Polyclonal |
Host Species | Goat |
Construction | Polyclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
Concentration | 1.0 mg/ml |
Research Background | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
Immunogen | Native Mouse IgG |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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