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Anti-CYP1A2 Antibody (9F602)
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Catalog No. TMAH-00328
Alias EC 1.14.14.1, Cytochrome P450-P3, Cytochrome P450 4, Cytochrome P450 1A2, Cytochrome P(3)450, CYPIA2, CYP1A2, Cholesterol 25-hydroxylase
Anti-CYP1A2 Antibody (9F602) is an antibody targeting CYP1A2. Anti-CYP1A2 Antibody (9F602) can be used in ELISA, WB, IHC.
Anti-CYP1A2 Antibody (9F602)
Anti-CYP1A2 Antibody (9F602)
😃Good
Catalog No. TMAH-00328Alias EC 1.14.14.1, Cytochrome P450-P3, Cytochrome P450 4, Cytochrome P450 1A2, Cytochrome P(3)450, CYPIA2, CYP1A2, Cholesterol 25-hydroxylase
Anti-CYP1A2 Antibody (9F602) is an antibody targeting CYP1A2. Anti-CYP1A2 Antibody (9F602) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CYP1A2 Antibody (9F602) is an antibody targeting CYP1A2. Anti-CYP1A2 Antibody (9F602) can be used in ELISA, WB, IHC. |
| Alias | EC 1.14.14.1, Cytochrome P450-P3, Cytochrome P450 4, Cytochrome P450 1A2, Cytochrome P(3)450, CYPIA2, CYP1A2, Cholesterol 25-hydroxylase |
| Ig Type | Rabbit IgG |
| Clone | 9F602 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, L02 whole cell lysate -All lanes: CYP1A2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 59 kDa -Observed band size: 59 kDa 2. IHC image of TMAH-00328 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation (Probable). |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Cytochrome P450 1A2 |
| Antigen Species | Human |
| Gene ID | 1544 |
| Uniprot ID | |
| Biology Area | Cancer, Cardiovascular, Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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