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Anti-Beta-Actin Antibody (5N615) (Synonyms: β-Actin Protein, β-Actin, Beta-Actin, Actin Cytoplasmic 1, ACTB)
Catalog No. TMAH-00020 Copy Product Info
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Anti-Beta-Actin Antibody (5N615) is an antibody targeting Beta-Actin. Anti-Beta-Actin Antibody (5N615) can be used in ELISA,FCM,WB.
Anti-Beta-Actin Antibody (5N615)
Copy Product Info🥰Excellent
Catalog No. TMAH-00020
Synonyms β-Actin Protein, β-Actin, Beta-Actin, Actin Cytoplasmic 1, ACTB
Anti-Beta-Actin Antibody (5N615) is an antibody targeting Beta-Actin. Anti-Beta-Actin Antibody (5N615) can be used in ELISA,FCM,WB.
Anti-Beta-Actin Antibody
(5N615)
(5N615)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $207 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Beta-Actin Antibody (5N615) is an antibody targeting Beta-Actin. Anti-Beta-Actin Antibody (5N615) can be used in ELISA,FCM,WB. |
| Synonyms | β-Actin Protein, β-Actin, Beta-Actin, Actin Cytoplasmic 1, ACTB |
| Ig Type | mIgG2b |
| Clone | 5N615 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in:U251 whole cell lysate (20µg), Hela whole cell lysate (20µg), HepG2 whole cell lysate (20µg), 293T whole cell lysate (20µg), A549 whole cell lysate (20µg), MCF7 whole cell lysate (20µg) -All lanes: ACTB antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/40000 dilution -Predicted band size:42 kDa -Observed band size:42 kDa -Exposure time:20s 2. Overlay Peak curve showing Hela cells stained with TMAH-00020 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Antimouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | |
| Recommended Dose | WB:1:500-1:2000; FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm, cytoskeleton. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ACTB Protein |
| Antigen Species | Human |
| Gene Name | ACTB |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Isotype/Loading Controls, Tags & Cell Markers, Signal transduction |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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