Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, WB, FCM

Reactivity: Human

mIgG2b

5N615

Human

1. Western Blot
-Positive WB detected in:U251 whole cell lysate (20µg), Hela whole cell lysate (20µg), HepG2 whole cell lysate (20µg), 293T whole cell lysate (20µg), A549 whole cell lysate (20µg), MCF7 whole cell lysate (20µg)
-All lanes: ACTB antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/40000 dilution
-Predicted band size:42 kDa
-Observed band size:42 kDa
-Exposure time:20s
2. Overlay Peak curve showing Hela cells stained with TMAH-00020 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Antimouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FCM

WB:1:500-1:2000; FCM:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human ACTB Protein

Human

60

Isotype/Loading Controls, Tags & Cell Markers, Signal transduction