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FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His)
(Synonyms: FcRn, Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha) Copy Product InfoSynonyms: FcRn, Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha
Catalog No. TMPY-02811 Copy Product Info
FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is Q61559&P01887.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $55 | - | In Stock | |
| 10 µg | $86 | - | In Stock | |
| 20 µg | $139 | - | In Stock | |
| 50 µg | $269 | - | In Stock | |
| 100 µg | $451 | - | In Stock | |
| 200 µg | $798 | 7-10 days | 7-10 days | |
| 500 µg | $1,690 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 98.6%; SEC-HPLC: 98.0%
Appearance:Lyophilized powder
Color:White
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Measured by its ability to bind human IgG1 in functional ELISA.
2.Loaded Mouse FcRn recombinant protein on His1k Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 8.3 nM as determined in a BLI assay.
3.Captured Mouse FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 32.9 nM as determined in an SPR assay (Biacore T200).
4.Captured Mouse FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 76.8 nM as determined in an SPR assay (Biacore T200).
5. The purity of Mouse FCGRT & B2M Heterodimer Protein is more than 95% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.
6.Loaded Recombinant Mouse FCGRT & B2M Heterodimer Protein, His Tag on His1K Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a with an affinity constant of 4.98nM as determined in BLI assay(QC tested). |
| Description | FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is Q61559&P01887. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | Q61559&P01887 |
| Construction | A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified Predicted N terminal: Ala 24 & Ile 21 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.  , |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | FcRn, Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha |
| Molecular Weight | 43.5 kDa (predicted); 35 & 12 kDa (reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His) chemical structure | FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His) molecular weight
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