1. The specific activity was determined to be＞70 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized EGF Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind  EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag)in functional ELISA.

EGFR Protein, Human, Recombinant (aa 668-1210, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 89.1 kDa and the accession number is P00533-1.

Human

Baculovirus Insect Cells

PIG61,NISBD2,mENA,HER1,ERBB1,ERBB,epidermal growth factor receptor

A DNA sequence encoding the cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Predicted N terminal: Met

> 75 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy