EGFR Protein, Human, Recombinant (aa 668-1210, His & GST)

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD
50 μg	In stock	$ 320.00
100 μg	5 days	$ 547.00
200 μg	5 days	$ 935.00
500 μg	5 days	$ 1,890.00

Pack Size

Availability

Price/USD

Quantity

50 μg

In stock

$ 320.00

100 μg

5 days

$ 547.00

200 μg

5 days

$ 935.00

500 μg

5 days

$ 1,890.00

Biological Information

1. The specific activity was determined to be＞70 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized EGF Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag)in functional ELISA.

Description

Species	Human
Expression System	Baculovirus-Insect Cells
Tag	His,GST
Accession Number	P00533-1
Synonyms	epidermal growth factor receptor, PIG61, ERBB1, NISBD2, ERBB, HER1, mENA
Construction	A DNA sequence encoding the cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 75 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 89.1 kDa

Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy