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Anti-NUF2 Antibody (2I629) is an antibody targeting NUF2. Anti-NUF2 Antibody (2I629) can be used in ELISA, WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB Reactivity: Human |
Alias | NUF2R, NUF 2, Kinetochore protein Nuf2, hsNuf2, hNuf2R, hNuf2, Cell division cycle-associated protein 1, CDCA1 |
Ig Type | Rabbit IgG |
Clone | 2I629 |
Reactivity | Human |
Verified Activity | Western Blot -Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate -All lanes: NUF2 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 55 kDa -Observed band size: 55 kDa |
Application | ELISA, WB |
Recommended Dose | WB:1:500-1:2000. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human NUF2 |
Antigen Species | Human |
Gene ID | 83540 |
Uniprot ID | |
Biology Area | Cell biology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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