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Synonyms: Stratum Corneum Tryptic Enzyme, SCTE, KLK-L2, KLK-5, KLK5, KLK 5, Kallikrein-Like Protein 2, Kallikrein-5


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $108 | 7-10 days | 7-10 days | |
| 10 µg | $169 | 7-10 days | 7-10 days | |
| 20 µg | $267 | 7-10 days | 7-10 days | |
| 50 µg | $488 | 7-10 days | 7-10 days | |
| 100 µg | $776 | 7-10 days | 7-10 days | |
| 200 µg | $1,230 | 7-10 days | 7-10 days | |
| 500 µg | $2,290 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1 μg/ml (100 μl/Well) on streptavidin (5 μg/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA (QC Test). |
| Description | Kallikrein 5/KLK5 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q9Y337. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q9Y337 |
| Construction | Ile67-Ser293 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 50 mM MES, 150 mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in 50 mM MES (pH 5.5). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | Stratum Corneum Tryptic Enzyme, SCTE, KLK-L2, KLK-5, KLK5, KLK 5, Kallikrein-Like Protein 2, Kallikrein-5 |
| Research Background | The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches. |
| Molecular Weight | 32.51 kDa (Predicted); 40-50 kDa (Reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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