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Synonyms: Stratum Corneum Tryptic Enzyme, SCTE, KLK-L2, KLK-5, KLK5, KLK 5, Kallikrein-Like Protein 2, Kallikrein-5


| Bioactivity | Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1 μg/ml (100 μl/Well) on streptavidin (5 μg/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA (QC Test). |
| Description | Kallikrein 5/KLK5 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q9Y337. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q9Y337 |
| Construction | Ile67-Ser293 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 50 mM MES, 150 mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in 50 mM MES (pH 5.5). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Stratum Corneum Tryptic Enzyme, SCTE, KLK-L2, KLK-5, KLK5, KLK 5, Kallikrein-Like Protein 2, Kallikrein-5 |
| Research Background |
| Molecular Weight | 32.51 kDa (Predicted); 40-50 kDa (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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