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Anti-c-Myc tag Polyclonal Antibody is a Rabbit antibody targeting c-Myc tag. Anti-c-Myc tag Polyclonal Antibody can be used in ELISA, ICC/IF, IP, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 40 μg | $305 | 7-10 days | 7-10 days |
| Description | Anti-c-Myc tag Polyclonal Antibody is a Rabbit antibody targeting c-Myc tag. Anti-c-Myc tag Polyclonal Antibody can be used in ELISA, ICC/IF, IP, WB. |
| Synonyms | cMyc tag |
| Ig Type | IgG |
| Specificity | c-Myc tagged fusion proteins |
| Verified Activity | 1. Western blot analysis of immunoprecipitates from c-Myc-tagged protein using Anti-c-Myc tag Polyclonal Antibody (TMAS-00137). Lane 1: c-Myc tagged fusion protein as control. Lane 2: Immunoprecipitates of c-Myc tagged fusion protein incubated with Rabbit IgG Control and Protein A served as negative control. Lane 3: Immunoprecipitates of c-Myc tagged fusion protein incubated with Anti-c-Myc tag Polyclonal Antibody (TMAS-00137) and Protein A. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Rabbit IgG. 2. Western blot analysis of c-Myc tagged fusion proteins expressed in E. coli cell lysate using Anti-c-Myc tag Polyclonal Antibody (TMAS-00137). The signal was developed with IRDyeTM800 Conjugated Goat Anti-Rabbit IgG. 3. Western blot analysis of c-Myc fusion protein (MW≈52KD) using 1 µg/ml Anti-c-Myc tag Polyclonal Antibody (TMAS-00137). Lane 1-3: c-Myc fusion protein in HEK293 cell lysate Lane 4: Negative HEK293 cell lysate Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody. |
| Application | |
| Recommended Dose | ELISA: 0.05-0.2 µg/ml; ICC/IF: 5-20 µg/ml; IP: 2-5 µg/ml; WB: 1:100-1:1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Purification | Affinity-chromatography |
| Appearance | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Formulation | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Research Background | The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. |
| Conjucates | Unconjugated |
| Immunogen | KLH conjugated synthetic peptide: EQKLISEEDL |
| Stability & Storage | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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