CD300A Protein, Human, Recombinant (hFc)

CD300A Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 44.5 kDa and the accession number is Q9UGN4-1.

Pack Size	Availability	Price/USD	Quantity
100 μg	5 days	$ 600.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 600.00

Description

CD300A Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 44.5 kDa and the accession number is Q9UGN4-1.

Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q9UGN4-1
Synonyms	CMRF-35H, CMRF-35-H9, CMRF35-H, IGSF12, CMRF35H, CMRF35-H9, CMRF35H9, CD300a molecule, IRC1, IRp60, IRC1/IRC2, CLM-8, IRC2
Construction	The Human CD300A (Q9UGN4-1)(Met1-Gln178) was expressed with the Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	44.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating ( CD300b, CD300e ) and inhibitory members ( CD300a, CD300f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD300c, CD300d ). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. It contains oneIg-like V-type ( immunoglobulin-like ) domain. CD300A / IGSF12 is an inhibitory receptor that may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).