FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPY-02830

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is Q8SPV9&Q8SPW0.

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPY-02830

Pack Size	Price	Availability
100 μg	$451	In Stock
200 μg	$798	7-10 days
500 μg	$1,690	7-10 days

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Product Information

Biological Activity	1. Measured by its ability to bind human IgG1 in functional ELISA. _x000D_ 2. Loaded Cynomolgus FcRn recombinant protein on His1K Biosensor, can bind IgG1 Fc with an affinity constant of 0.032 μM as determined in a BLI assay. _x000D_ 3. Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.09 µM as determined in an SPR assay. _x000D_ 4. Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.18 µM as determined in an SPR assay (Biacore T200). _x000D_ 5. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein is more than 95% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.
Description	FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is Q8SPV9&Q8SPW0.
Species	Cynomolgus
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q8SPV9&Q8SPW0
Synonyms	Fc fragment of IgG, receptor, transporter, α,Fc fragment of IgG, receptor, transporter, alpha
Construction	A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of cynomolgus FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. Predicted N terminal: Ala 24 & Ile 21
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Molecular Weight	43.5 kDa (predicted); 35 & 12 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.