FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 with His tag. The predicted molecular weight is 43.5 kDa. Accession number: Q8SPV9&Q8SPW0
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | In stock | $ 451.00 | |
200 μg | 5 days | $ 798.00 | |
500 μg | 5 days | $ 1,690.00 |
Biological Information |
1. Measured by its ability to bind human IgG1 in functional ELISA. 2. Loaded Cynomolgus FcRn recombinant protein on His1K Biosensor, can bind IgG1 Fc with an affinity constant of 0.032 μM as determined in a BLI assay. 3. Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.09 µM as determined in an SPR assay. 4. Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.18 µM as determined in an SPR assay (Biacore T200). 5. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein is more than 95% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.
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Description | FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 with His tag. The predicted molecular weight is 43.5 kDa. Accession number: Q8SPV9&Q8SPW0 |
Species | Cynomolgus |
Expression System | HEK293 |
Tag | His |
Accession Number | Q8SPV9&Q8SPW0 |
Synonyms | Fc fragment of IgG, receptor, transporter, alpha, Fc fragment of IgG, receptor, transporter, α |
Construction | A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of cynomolgus FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. |
Protein Purity |
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
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Molecular Weight | Approxiamtely 43.5 kDa |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
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Please read the User Guide of Recombinant Proteins for more specific information.
FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His) Fc fragment of IgG, receptor, transporter, alpha Fc fragment of IgG, receptor, transporter, α recombinant recombinant-proteins proteins protein