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Anti-GST tag Antibody (4G528) is a Mouse antibody targeting GST tag. Anti-GST tag Antibody (4G528) can be used in ELISA, IP, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μg | $462 | 7-10 days | 7-10 days |
| Description | Anti-GST tag Antibody (4G528) is a Mouse antibody targeting GST tag. Anti-GST tag Antibody (4G528) can be used in ELISA, IP, WB. |
| Synonyms | GSTtag |
| Ig Type | IgG1,κ |
| Clone | 4G528 |
| Specificity | GST localized at the C-terminal, N-terminal, and internal region of GST-tagged fusion proteins |
| Application | |
| Recommended Dose | ELISA: 0.05-0.2 µg/ml; IP: 1 µg/ml; WB: 0.1-1.0 µg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Purification | Protein A affinity column |
| Appearance | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| Formulation | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Research Background | Schistosoma japonicum glutathione S-transferase (GST) is a 26 kDa enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E.coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest. |
| Conjucates | Unconjugated |
| Others Formats | HRP |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: GST protein |
| Stability & Storage | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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