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Anti-MBP tag Antibody (8B845)
😃Good
Catalog No. TMAB-01111
Anti-MBP tag Antibody (8B845) is a Mouse antibody targeting MBP tag. Anti-MBP tag Antibody (8B845) can be used in WB.
Anti-MBP tag Antibody (8B845)
Anti-MBP tag Antibody (8B845)
😃Good
Catalog No. TMAB-01111
Anti-MBP tag Antibody (8B845) is a Mouse antibody targeting MBP tag. Anti-MBP tag Antibody (8B845) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $127 | 7-10 days | |
| 100 μL | $182 | 7-10 days | |
| 500 μL | $754 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MBP tag Antibody (8B845) is a Mouse antibody targeting MBP tag. Anti-MBP tag Antibody (8B845) can be used in WB. |
| Clone | 8B845 |
| Reactivity | Species independent |
| Verified Activity | 100 ng rhC-jun-His-MBP proteinper Lane probed with MBP tag monoclonal antibody respectively, unconjugated (TMAB-01111) at 1:10000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB |
| Recommended Dose | WB: 1:1000-10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Periplasm. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein. |
| Immunogen | Recombinant Protein: MBP Protein of Escherichia coli (strain K12) |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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