Anti-CEACAM5 Antibody (1V100) is an antibody targeting CEACAM5. Anti-CEACAM5 Antibody (1V100) can be used in ELISA, IF, FC.

Rabbit IgG

1V100

Human

1. Immunofluorescence staining of Hela cell with TMAH-00260 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).
2. Immunofluorescence staining of MCF7 cell with TMAH-00260 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).
3. Overlay Peak curve showing Hela cells surface stained with TMAH-00260 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was human IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,026 events was performed.

ELISA, IF, FC

IF:1:20-1:200; FC:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human CEACAM5 Protein

Human

1048

Cancer, Tags & Cell Markers, Immunology, Stem cells