Anti-CD68 Polyclonal Antibody 5 is a Rabbit antibody targeting CD68. Anti-CD68 Polyclonal Antibody 5 can be used in FCM,ELISA.

IgG

Human,Mouse (predicted:Rat)

1. Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag (Mouse CD68 Antibody, the EC50 is 175.4 ng/mL.
2. Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag (Mouse CD68 Antibody, the EC50 is 142.5 ng/mL.
3. Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD68 antibody (TMAB-04053)
Dilution: 1 ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. The THP-1 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CD68 antibody (TMAB-04053): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM: 1μg/Test; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: mouse CD68 protein

Mouse

CD68

Macrosialin

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.