TSLP Protein, Human, Recombinant (His), Biotinylated

1.Immobilized Recombinant Human TSLP Protein (R127A, R130S, His Tag), Biotinylated (Cat#TMPY-05756) at 2 μg/mL (100 μl/well) on Streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human CRLF2 Protein (Fc Tag) (Cat#TMPY-05769), the EC50 is 8-30 ng/mL (QC tested).
2.Loaded Recombinant Human CRLF2/TSLPR Protein (hFc Tag) (Cat#TMPY-05769) on proA Biosensor, can bind Biotinylated Recombinant Human TSLP Protein (R127A, R130S, His Tag) (Cat#TMPY-05756) with an affinity constant of 42.000 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

TSLP Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.2 kDa and the accession number is Q969D9-1.

Human

HEK293 Cells

> 95 % as determined by SDS-PAGE.

Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like cytokine originally characterized by its ability to promote the activation of B cells and dendritic cells (DCs). Thymic stromal lymphopoietin (TSLP) is a cytokine expressed by epithelial cells, including keratinocytes, and is important in allergic inflammation. Subsequent studies have shown that TSLP promotes T helper type 2 (TH2) cell responses associated with immunity to some helminth parasites and the pathogenesis of many inflammatory diseases, including atopic dermatitis and asthma. TSLP can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of DC-derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues.