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Anti-TOMM22 Antibody (6B293) is an antibody targeting TOMM22. Anti-TOMM22 Antibody (6B293) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
Alias | Translocase of outer membrane 22 kDa subunit homolog, TOMM 22, TOM22, Mitochondrial import receptor subunit TOM22 homolog, hTom22, 1C9-2 |
Ig Type | Rabbit IgG |
Clone | 6B293 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate -All lanes: TOMM22 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 16 kDa -Observed band size: 18 kDa 2. IHC image of TMAH-01196 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human TOMM22 |
Antigen Species | Human |
Gene ID | 56993 |
Uniprot ID | |
Biology Area | Other |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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