EphB3 Protein, Human, Recombinant (His)

(Synonyms: TYRO6, Sek4, MDK5, HEK2, ETK2, EPHB3, EK2, EC 2.7.10.1, EC 2.7.10, Cek10, AW456895) Copy Product Info

Synonyms: TYRO6, Sek4, MDK5, HEK2, ETK2, EPHB3, EK2, EC 2.7.10.1, EC 2.7.10, Cek10, AW456895

Catalog No. TMPK-00898 Copy Product Info

Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.

Pack Size	Price	USA Stock	Global Stock
5 μg	$51	7-10 days	7-10 days
10 μg	$80	7-10 days	7-10 days
20 μg	$129	7-10 days	7-10 days
50 μg	$248	7-10 days	7-10 days
100 μg	$418	7-10 days	7-10 days
200 μg	$757	7-10 days	7-10 days
500 μg	$1,670	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human EphB3, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1 ng/mL determined by ELISA.(QC Test)
Description	Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P54753
Construction	Gly34-Leu559
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonyms	TYRO6, Sek4, MDK5, HEK2, ETK2, EPHB3, EK2, EC 2.7.10.1, EC 2.7.10, Cek10, AW456895
Research Background

Chemical Properties

Molecular Weight

58.6 kDa (predicted). Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

TYRO-6TYRO 6MDK-5MDK 5HEK-2HEK 2ETK-2ETK 2EPHB-3EPHB 3EK-2EK 2AW-456895AW 456895

Related Tags: EphB3 Protein, Human, Recombinant (His) chemical structure | EphB3 Protein, Human, Recombinant (His) molecular weight