Siglec-10 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 100-130 KDa and the accession number is Q96LC7.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 μg | 5 days | $ 355.00 | |
100 μg | 5 days | $ 963.00 |
Description | Siglec-10 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 100-130 KDa and the accession number is Q96LC7. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-hFc-Avi |
Accession Number | Q96LC7 |
Synonyms | siglec-like gene 2, SLG2, sialic acid-binding Ig-like lectin 10, Siglec-like protein 2, MGC126774, Siglec-10, SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7, PRO940, SIGLEC10 |
Amino Acid | Met17Thr546 |
Construction | Met17Thr546 |
Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Molecular Weight | 100-130 KDa (reducing condition) |
Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products. |
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Siglec-10 Protein, Human, Recombinant (hFc & Avi), Biotinylated siglec-like gene 2 SLG2 sialic acid-binding Ig-like lectin 10 SIGLEC 10 Siglec-like protein 2 MGC126774 MGC-126774 Siglec-10 SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7 PRO 940 SIGLEC-10 PRO940 MGC 126774 PRO-940 SIGLEC10 SLG-2 SLG 2 recombinant recombinant-proteins proteins protein