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Anti-RAB27A Antibody (3T522)
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Catalog No. TMAH-01030
Alias Ras-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram
Anti-RAB27A Antibody (3T522) is an antibody targeting RAB27A. Anti-RAB27A Antibody (3T522) can be used in ELISA, FCM.
Anti-RAB27A Antibody (3T522)
Anti-RAB27A Antibody (3T522)
😃Good
Catalog No. TMAH-01030Alias Ras-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram
Anti-RAB27A Antibody (3T522) is an antibody targeting RAB27A. Anti-RAB27A Antibody (3T522) can be used in ELISA, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-RAB27A Antibody (3T522) is an antibody targeting RAB27A. Anti-RAB27A Antibody (3T522) can be used in ELISA, FCM. |
| Synonyms | Ras-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram |
| Ig Type | Rabbit IgG |
| Clone | 3T522 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing PC3 cells stained with TMAH-01030 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | |
| Recommended Dose | FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. |
| Tissue Specificity | Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human RAB27A |
| Antigen Species | Human |
| Gene Name | RAB27A |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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