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Anti-RAB27A Antibody (3T522)

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Catalog No. TMAH-01030
Alias Ras-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram

Anti-RAB27A Antibody (3T522) is an antibody targeting RAB27A. Anti-RAB27A Antibody (3T522) can be used in ELISA, FCM.

Anti-RAB27A Antibody (3T522)

Anti-RAB27A Antibody (3T522)

😃Good
Catalog No. TMAH-01030Alias Ras-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram
Anti-RAB27A Antibody (3T522) is an antibody targeting RAB27A. Anti-RAB27A Antibody (3T522) can be used in ELISA, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, FCM

Reactivity: Human
AliasRas-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram
Ig Type
Rabbit IgG
Clone
3T522
Reactivity
Human
Verified Activity
Overlay Peak curve showing PC3 cells stained with TMAH-01030 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, FCM
Recommended Dose
FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationMembrane; Lipid-anchor. Melanosome. Late endosome. Lysosome.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundSmall GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human RAB27A
Antigen Species
Human
Gene ID
5873
Uniprot ID
Biology Area
Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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