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Anti-RAB27A Antibody (3T522) is an antibody targeting RAB27A. Anti-RAB27A Antibody (3T522) can be used in ELISA, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, FCM Reactivity: Human |
Alias | Ras-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram |
Ig Type | Rabbit IgG |
Clone | 3T522 |
Reactivity | Human |
Verified Activity | Overlay Peak curve showing PC3 cells stained with TMAH-01030 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, FCM |
Recommended Dose | FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human RAB27A |
Antigen Species | Human |
Gene ID | 5873 |
Uniprot ID | |
Biology Area | Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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