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Anti-CD20 Polyclonal Antibody

😃Good
Catalog No. TMAB-00341

Anti-CD20 Polyclonal Antibody is a Rabbit antibody targeting CD20. Anti-CD20 Polyclonal Antibody can be used in WB,FCM,ELISA.

Anti-CD20 Polyclonal Antibody

Anti-CD20 Polyclonal Antibody

😃Good
Catalog No. TMAB-00341
Anti-CD20 Polyclonal Antibody is a Rabbit antibody targeting CD20. Anti-CD20 Polyclonal Antibody can be used in WB,FCM,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD20 Polyclonal Antibody is a Rabbit antibody targeting CD20. Anti-CD20 Polyclonal Antibody can be used in WB,FCM,ELISA.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat)
Verified Activity
1. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD20 antibody (TMAB-00341), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00341, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
2. Sample:
Lane 1: LympHnode (Mouse) Lysate at 40 μg
Lane 2: Bone (Mouse) Lysate at 40 μg
Primary: Anti-CD20 (TMAB-00341) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Application
WB,FCM,ELISA
Recommended Dose
WB: 1:500-2000; FCM: 1μg /Test; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD20
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Tumor-associated antigens,CD,Human Lineage Negative
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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