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Anti-CD20 Polyclonal Antibody is a Rabbit antibody targeting CD20. Anti-CD20 Polyclonal Antibody can be used in WB,FCM,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-CD20 Polyclonal Antibody is a Rabbit antibody targeting CD20. Anti-CD20 Polyclonal Antibody can be used in WB,FCM,ELISA. |
Ig Type | IgG |
Reactivity | Mouse (predicted:Human,Rat) |
Verified Activity | 1. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD20 antibody (TMAB-00341), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00341, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. 2. Sample: Lane 1: LympHnode (Mouse) Lysate at 40 μg Lane 2: Bone (Mouse) Lysate at 40 μg Primary: Anti-CD20 (TMAB-00341) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa |
Application | WB,FCM,ELISA |
Recommended Dose | WB: 1:500-2000; FCM: 1μg /Test; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. |
Immunogen | KLH conjugated synthetic peptide: human CD20 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Tumor-associated antigens,CD,Human Lineage Negative |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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