Anti-CD171/L1CAM Polyclonal Antibody 2 is a Rabbit antibody targeting CD171/L1CAM. Anti-CD171/L1CAM Polyclonal Antibody 2 can be used in FCM,ELISA.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Antigen: NCAM-L1/CD171 Antibody Blocking Peptide, 0.2 μg/100 μl
Primary: Antiserum, 1:500, 1:1000, 1: 2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;
TMB staining;
Read the data in MicroplateReader by 450 nm.
2. Antigen: NCAM-L1/CD171 Antibody Blocking Peptide, 0.2 μg/100 μl
Primary: Antiserum, 1:500, 1:1000, 1: 2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 5000;
TMB staining;
Read the data in MicroplateReader by 450 nm.
3. Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD171 antibody (TMAB-03879), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA.
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions).
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.

FCM: 1μg/Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human CD171

Human

L1CAM

Neural cell adhesion molecule L1

Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.